PubMed · 10892097
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P Keto, A Haapanen, T Kallio. 1997. [Visualizing the arteries].. https://pubmed.ncbi.nlm.nih.gov/10892097/
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Identification of factors that may stimulate ischemia-induced neovascularization without increasing atherosclerotic plaque progression is of major therapeutic importance. We hypothesized that interleukin-18 binding protein (IL-18BP), a major antiinflammatory protein with plaque-stabilizing activities, may affect the neovascularization in mice ischemic hindlimb. Ischemia was produced by artery femoral occlusion in mice that were subjected to in vivo intramuscular electrotransfer of either an empty plasmid or a murine IL-18BP plasmid. Angiographic score, capillary density (CD31 staining), and laser Doppler perfusion data at day 28 showed significant improvement in ischemic/nonischemic leg ratio by respectively 1.6-, 1.4-, and 1.5-fold in IL-18BP-treated mice compared with controls (P<0.01). This was associated with a significant 2-fold increase in both vascular endothelial growth factor (VEGF) and phospho-Akt protein content in the ischemic hindlimb of IL-18BP-treated mice (P<0.05). Similar results were obtained in IL-18-deficient mice. Because bone marrow-derived endothelial progenitor cells (BM-EPCs) are involved in postnatal vasculogenesis, EPCs were isolated and cultivated from bone marrow mononuclear cells. IL-18BP treatment led to a significant 1.8-fold increase in the percentage of BM-EPCs characterized as cells positive for both AcLDL-Dil and von Willebrand factor (P<0.001). In conclusion, IL-18BP stimulates ischemia-induced neovascularization in association with an activation of VEGF/Akt signaling and an increase in BM-EPCs mobilization and differentiation. Our findings strongly suggest a major antiangiogenic role of endogenous IL-18 in postischemic injury.
Vascular anomalies can cause epistaxis in children. The authors present two cases of recurrent epistaxis secondary to rare causes and discuss their presentations. Both cases were managed conservatively since intervention in these vascular lesions carries a high risk of damaging adjacent structures.
Data on vascular anatomy traditionally have been derived from time-consuming gross dissection and histology, which has prevented the assembly of large sample sizes. Vascular injection of radiopaque medium (angiography) is a rapid technique, but, as typically performed, it has limitations (e.g., superimposition and poor subsequent dissectibility). We present a novel angiographic technique comprised of two elements: 1) a new, dissectible injection medium; and (2) stereoradiography. The injection medium consists of liquid barium (providing radiopacity) and latex (providing dissectibility). Domestic duck heads were the study system. The relative concentrations of barium and latex were varied, and the resulting radiographs were assayed for vessel radiopacity and the number of observable vessels. A wide range of barium percentages yielded excellent results, suggesting that preparation of the medium can be "eyeballed" for most applications, which enhances processing speed. The stereoradiographic element solved the superimposition problem, allowing stunning resolution of the spatial relationships of vessels to each other and to other tissues. Stereoangiography is a fast and easy technique that permits the acquisition of detailed anatomical data from many specimens, thereby enabling something rarely achieved: population-level anatomical studies.