A monoclonal IgG4 (lambda) with factor V inhibitory activity.

P E Hurtubise; M C Coots; D J Jacob; A F Muhleman; H I Glueck

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PubMed · 109525

A monoclonal IgG4 (lambda) with factor V inhibitory activity.

P E Hurtubise·M C Coots·D J Jacob·A F Muhleman·H I Glueck

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Abstract

A monoclonal IgG4 (lambda) with inhibitory activity to human coagulation factor V was isolated from the serum of a patient with a fatal hemorrhagic diathesis by using a combination of DE-52 ion exchange chromatography and isoelectric focusing techniques. Using the criteria for defining a monoclonal immunoglobulin of restricted mobility on protein electrophoresis, immunoelectrophoresis, and isoelectric focusing, as well as neutralization with class, subclass, and light chain type antisera, we are the first to demonstrate a factor V inhibitor as a monoclonal IgG4 (lambda) detectable in serum or plasma.

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Chromatography, Ion Exchange Clone Cells Factor V Humans Immune Sera Immunoglobulin G Immunoglobulin lambda-Chains Isoelectric Focusing Neutralization Tests Journal Article

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P E Hurtubise, M C Coots, D J Jacob, A F Muhleman, H I Glueck. 1979. A monoclonal IgG4 (lambda) with factor V inhibitory activity.. https://pubmed.ncbi.nlm.nih.gov/109525/

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