Current diagnostic techniques for pulmonary embolism.

J G Weg

Home/Archive/PubMed

PubMed · 11005461

Current diagnostic techniques for pulmonary embolism.

J G Weg

View original source ↗Export metadata ↓

Abstract

The diagnosis of venous thromboembolism (VTE) includes deep venous thrombosis (DVT) and pulmonary embolism (PE) and requires objective testing. The clinician uses a combination of risk factors and nonspecific clinical findings to identify patients who warrant such an evaluation. The recommended approach begins with ventilation/perfusion (V/Q) lung scans or lower extremity noninvasive studies by compression ultrasonography. Nondiagnostic V/Q scans or negative noninvasive studies require further testing. A high-probability V/Q scan or a positive noninvasive study warrant treatment. A normal V/Q scan excludes the diagnosis of PE. Helical computed tomography (CT) can diagnose PE of major vessels but is not sufficiently sensitive to exclude PE because of its poor sensitivity for subsegmental pulmonary vessels. Newer D-dimer assays have a high negative predictive value, but results vary with the specific assay and do not perform well in patients with cancer. Future studies are needed to validate magnetic resonance imaging (MRI) or magnetic resonance angiography (MRA).

Explore related subjects

Humans Leg Lung Magnetic Resonance Angiography Magnetic Resonance Imaging Predictive Value of Tests Pulmonary Embolism Reproducibility of Results Risk Factors Sensitivity and Specificity Tomography, X-Ray Computed Ultrasonography, Doppler, Color Venous Thrombosis Ventilation-Perfusion Ratio Journal Article Review

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

J G Weg. 2000. Current diagnostic techniques for pulmonary embolism.. https://pubmed.ncbi.nlm.nih.gov/11005461/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2027

Global Genomic Surveillance.

Global genomic surveillance has emerged as a foundational pillar of public health in the twenty-first century, enabling real-time tracking of pathogen evolution and informing outbreak response. This chapter examines the strategic architecture of global genomic surveillance, focusing on its application to arboviruses such as chikungunya virus (CHIKV). It explores the integration of genomic data with epidemiological, clinical, and environmental information within a One Health framework, while addressing critical challenges in governance, equity, and interoperability. The discussion covers the entire genomic surveillance workflow, from sample collection and sequencing to bioinformatic analysis and phylogenetic inference, and highlights the transformative role of artificial intelligence (AI) in predictive surveillance. By analyzing global initiatives, operational barriers, and emerging technologies, this chapter underscores the necessity of sustainable, equitable, and interoperable genomic systems to proactively address current and future infectious disease threats.

Humans↗

PubMed2027

Systematic Dissection of Key Driver Perturbation Signatures in Single Cells via ECCITE-seq.

CRISPR screens, such as expanded CRISPR-compatible cellular indexing of transcriptomes and epitopes by sequencing (ECCITE-seq), enable the simultaneous measurement of transcriptomes, gRNA identity, and cell-surface protein expression at single-cell resolution to systematically interrogate gene function. This platform provides a powerful and scalable experimental approach for validating disease-associated regulators identified by large-scale association studies and other computational methods, including network-based analyses of multi-omics data. Here, as an example application, we describe an ECCITE-seq framework to characterize the transcriptomic consequences of perturbing multiple neuronal key driver genes associated with Alzheimer's disease (AD) in human-induced pluripotent stem cell (hiPSC)-derived neurons. More broadly, by integrating customized pooled gRNA libraries with different CRISPR effectors across multiple cell types, this approach allows for the assessment of the regulatory impact of candidate genes implicated in development and disease processes.

Humans↗

PubMed2027

Identification of Genome-Wide Chromatin Structural Aberration in Cancer by Hi-C Analysis.

Aberrant three-dimensional genome organization is a hallmark of cancer, often driving oncogene activation through mechanisms such as enhancer hijacking. High-throughput chromosome conformation capture (Hi-C) maps these interactions on a genome-wide scale. Unlike earlier dilution-based methods, in situ Hi-C performs proximity ligation within intact nuclei, minimizing random ligation noise and enabling fine-scale structure detection. This chapter describes an optimized in situ Hi-C protocol tailored for cancer cell lines using MboI digestion and biotin-mediated pull-down to generate high-complexity libraries. We further outline a computational workflow that extends beyond standard topological mapping of compartments and topologically associating domains to identify cancer-specific aberrations. Specifically, we focus on detecting chromosomal rearrangements (structural variants) and characterizing the distinct circular topology of extrachromosomal DNA. This integrated experimental and analytical framework provides the necessary tools to dissect the spatial dysregulation underlying tumor evolution.

Humans↗