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Coherent scattering in multi-harmonic light microscopy.

L Moreaux·O Sandre·S Charpak·M Blanchard-Desce·J Mertz
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Abstract

By focusing a pulsed laser beam into a sample, harmonic up-conversion can be generated as well as multi-photon excited fluorescence. Whereas multi-photon excited fluorescence microscopy is well established, the use of multi-harmonic generation for three-dimensional image contrast is very recent. Both techniques can provide similar resolution and, for adequate radiating source density, comparable signal levels, allowing them to be combined in a single versatile instrument. However, harmonic generation differs fundamentally from fluorescence generation in that it is coherent and produces radiation patterns that are highly sensitive to phase. As such, multi-harmonic generation microscopy provides a unique window into molecular spatial organization that is inaccessible to fluorescence.

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AnimalsCell MembraneFluorescent DyesHippocampusIn Vitro TechniquesLasersLightMicroscopy, FluorescencePyramidal CellsPyridinium CompoundsScattering, RadiationSensitivity and SpecificityComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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L Moreaux, O Sandre, S Charpak, M Blanchard-Desce, J Mertz. 2001. Coherent scattering in multi-harmonic light microscopy.. https://doi.org/10.1016/s0006-3495(01)76129-2

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