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PubMed · 11236706

[Fluorescent mRNA differential display technique].

G S Wang·M W Wang·W D You·H F Wang·M F Feng
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Abstract

AIM: To apply fluorescent mRNA differential display technique. METHODS: Total RNA samples were extracted from human monocyte line U937 treated/untreated with IFN and LPS, and were used as templates in differential display PCR. The anchored primers used were labeled with the fluorescent tag. After running on 5.6% denaturing PAGE gel, differentially expressed bands were excised and recovered, and finally reamplified. RESULTS: Three tested samples all showed amplified bands differed from 300 bp to 2.0 kb, the bands were bright and clear, the background was low. Both yes/no changes and upregulated/downregulated happenings were shown simultaneously. The reamplification bands were sharp and pure. CONCLUSION: We have successfully practiced fluorescent differential display technique in our lab. It is a fast, safe and cost-effective method used to sereen unknown expressed genes.

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Cell LineFluorescenceGene Expression ProfilingHumansMonocytesEnglish AbstractJournal ArticleResearch Support, Non-U.S. Gov't

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G S Wang, M W Wang, W D You, H F Wang, M F Feng. 2000. [Fluorescent mRNA differential display technique].. https://pubmed.ncbi.nlm.nih.gov/11236706/

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