Effect of serial passage on gene expression in MC3T3-E1 preosteoblastic cells: a microarray study.

W Huang; B Carlsen; G H Rudkin; N Shah; C Chung; K Ishida; D T Yamaguchi; T A Miller

doi:10.1006/bbrc.2001.4458

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Effect of serial passage on gene expression in MC3T3-E1 preosteoblastic cells: a microarray study.

W Huang·B Carlsen·G H Rudkin·N Shah·C Chung·K Ishida·D T Yamaguchi·T A Miller

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Abstract

The osteoblastic function of mouse preosteoblastic MC3T3-E1 cells, as measured by alkaline phosphatase activity and osteocalcin secretion, decreases after serial passage. To uncover genes responsible for decreased osteoblastic function in high-passage cells, we have studied passage-dependent change of gene expression in MC3T3-E1 cells. Changes in the expression pattern of 2000 selected genes were examined simultaneously by comparing mRNA levels between MC3T3-E1 cells at passage 20 and passage 60 using the cDNA microarray analysis. Significant changes in the steady-state abundance of 27 mRNAs were observed in response to different passage numbers, including 17 known genes, 4 ESTs with homology to known genes, and 6 genes with no previously described function or homology. Northern blot analysis was used to verify and quantify the expression of selected genes, and revealed a significant higher level of up- and down-regulation compared to microarray data. These results indicate the existence of a significant change in gene expression in osteoblastic cells undergoing serial passages. Such changes might be responsible for a reduction in bone regeneration in older osteoblasts. Potential roles of selected genes in bone aging are discussed.

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Aging Animals Blotting, Northern Cell Line Gene Expression Profiling Mice Oligonucleotide Array Sequence Analysis Osteoblasts RNA, Messenger Stem Cells Transcription, Genetic Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

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W Huang, B Carlsen, G H Rudkin, N Shah, C Chung, K Ishida, D T Yamaguchi, T A Miller. 2001. Effect of serial passage on gene expression in MC3T3-E1 preosteoblastic cells: a microarray study.. https://doi.org/10.1006/bbrc.2001.4458

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