PubMed · 11273094
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T J John. 2001-02-10. Difficulties in polio eradication.. https://doi.org/10.1016/s0140-6736(05)71283-6
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Sulfoquinovose (SQ), a sulfonated sugar produced on a gigaton scale each year, contributes to global sulfur cycling, yet the microbes and pathways mediating its turnover in the environment have been inferred largely from genomic potential rather than direct activity. Here, we coupled incubations of environmental samples with 13C-labeled SQ to DNA-stable isotope probing to identify active SQ carbon assimilators across estuary, mangrove, and lake ecosystems. In estuarine communities, Vibrio and Cognatishimia incorporated SQ-derived 13C; Novosphingobium dominated in the mangrove, and Agrobacterium in the lake. Pure-culture experiments, coupled with comparative proteomics and gene knockout validation, demonstrated that Vibrio strains degrade SQ via modified sulfoglycolytic Embden-Meyerhof-Parnas and Entner-Doudoroff pathways to produce the environmentally significant organosulfur 2,3-dihydroxypropanesulfonate. Comparative genomic analyses suggested that closely related genome representatives of Novosphingobium, Cognatishimia, and Agrobacterium encode the sulfolytic SQ monooxygenase pathway. A global survey of aquatic microbial genomes indicated that over 9% harbor SQ degradation clusters, supporting a widespread distribution of bacterial SQ catabolic potential in aquatic environments.
A 250 km2 area of the Permo-Triassic Sandstone aquifer in the West Midlands of England, UK, was selected as a test region for the development of a geographic information system (GIS)-based risk assessment methodology that incorporates contaminant source, groundwater vulnerability and groundwater abstraction catchment elements in order to prioritise areas and boreholes potentially at risk from chlorinated solvent pollution on a regional scale. Factors incorporated in the vulnerability assessment include the nature of soils, presence or absence of superficial or glacial deposits, fault density and depth to water table. ARCVIEW GIS was employed with a simple ranking system from which the derived vulnerability assessment index was combined with current chlorinated solvent user industry data and source protection zone components. Results indicate the presence of high-risk areas in urban locations where locally dense distributions of chlorinated solvent user industries combine with high vulnerability aquifers within the catchment of supply boreholes. Ranking of catchment-specific risk reveals the abstraction points under greatest stress. The proposed methodology has applications as a regional-scale initial screening tool to guide site selection for regulatory inspections and assist in prioritising monitoring strategies for existing boreholes. Future developments will provide guidance for locating new urban boreholes in areas of lowest risk.
The dimensionless Henry's law constant was determined for methyl tert-butyl ether (MTBE) at six temperatures (3, 5, 10, 15, 20 and 25 degrees C) by using a thermostatted flask (430 ml) containing an aqueous MTBE solution. The ratio between the gas phase and the water phase in the flask was approximately 1.7:1. The aim of this study was to acquire data needed to model the behaviour of MTBE at groundwater conditions. The dimensionless Henry's law constant at 10 degrees C is approximately 0.01 but is 0.03 at 25 degrees C. This is important for modelling MTBE because the variation of vaporization cannot be disregarded at groundwater temperatures. In a second experiment the water solubility of MTBE was determined to be 62.1 g/l at 5 degrees C and 35.5 g/l at 20 degrees C). The high solubility at low temperatures could cause MTBE plumes from spills (fuel accidents etc.) to spread rapidly.