Cloning of a cDNA encoding EIN3-like protein (DC-EIL1) and decrease in its mRNA level during senescence in carnation flower tissues.

K Waki; K Shibuya; T Yoshioka; T Hashiba; S Satoh

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PubMed · 11283184

Cloning of a cDNA encoding EIN3-like protein (DC-EIL1) and decrease in its mRNA level during senescence in carnation flower tissues.

K Waki·K Shibuya·T Yoshioka·T Hashiba·S Satoh

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Abstract

A cDNA clone encoding a putative EIN3-like protein (DC-EIL1) was obtained from total RNA isolated from senescing carnation (Dianthus caryophyllus L.) petals using RT-PCR and RACE techniques. The cDNA (2382 bp) contained an open reading frame of 1986 bp corresponding to 662 amino acids. The amino acid sequence of the N-terminal half of the protein, ranging from 80-300 amino acid residues, had 84% identity with that of the corresponding regions of Arabidopsis EIN3 and tobacco TEIL, although the overall identity was 49% and 52%, respectively. Northern blot analysis revealed that the amount of mRNA corresponding to DC-EIL1 decreased in flower tissues, especially in petals, during natural senescence and senescence induced by exogenously applied ethylene or ABA.

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Amino Acid Sequence Arabidopsis Proteins Blotting, Northern Cellular Senescence Cloning, Molecular DNA, Plant DNA-Binding Proteins Magnoliopsida Molecular Sequence Data Nuclear Proteins Plant Proteins Plant Stems RNA, Messenger RNA, Plant Sequence Alignment Transcription Factors Journal Article

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K Waki, K Shibuya, T Yoshioka, T Hashiba, S Satoh. 2001. Cloning of a cDNA encoding EIN3-like protein (DC-EIL1) and decrease in its mRNA level during senescence in carnation flower tissues.. https://pubmed.ncbi.nlm.nih.gov/11283184/

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