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Trimethylamine and total volatile basic nitrogen determination by flow injection/gas diffusion in Mediterranean hake (Merluccius merluccius).

S Baixas-Nogueras·S Bover-Cid·M C Vidal-Carou·M T Veciana-Nogués·A Mariné-Font
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Abstract

The reliability of flow injection/gas diffusion (FIGD) methods to determine trimethylamine (TMA-N) and total volatile basic nitrogen (TVB-N) in hake was studied in order to find an alternative and accurate, simple, cheap, and rapid method for non-protein nitrogen determination. FIGD methods involved extracting volatile amines with 7.5% trichloroacetic acid, followed by the injection of the extracts into the FIGD manifold, previously adjusted for TMA-N or TVB-N determinations. Each determination took approximately 2 min. Reliability was satisfactory in linearity, precision, recovery, and sensitivity. There was good correlation (p < 0.001) between FIGD and the classic official methods, for both TMA-N and TVB-N determinations, and also between FIGD and the gas chromatographic procedure described for TMA-N. These results proved that FIGD methods are simpler, cheaper, and faster than current official procedures. To check the suitability of FIGD procedures over a wide range of analyte concentrations, changes of both TMA-N and TVB-N and the P ratio values throughout the ice storage of hake were monitored. The usefulness of each of these potential freshness indicators for hake is discussed.

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AnimalsDiffusionFishesFlow Injection AnalysisFood PreservationMethylaminesNitrogenSeafoodSensitivity and SpecificityVolatilizationJournal Article

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S Baixas-Nogueras, S Bover-Cid, M C Vidal-Carou, M T Veciana-Nogués, A Mariné-Font. 2001. Trimethylamine and total volatile basic nitrogen determination by flow injection/gas diffusion in Mediterranean hake (Merluccius merluccius).. https://doi.org/10.1021/jf000649n

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