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PubMed · 11325894

Relationship between triglyceride concentrations and LDL size evaluated by malondialdehyde-modified LDL.

A Kondo·Y Muranaka·I Ohta·K Notsu·M Manabe·K Kotani·K Saito·M Maekawa·T Kanno
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Abstract

BACKGROUND: Hypertriglyceridemia is associated with decreased HDL-cholesterol (HDL-C) and increased small dense LDL. In addition, small dense LDL is known to be susceptible to oxidation. METHODS: We measured LDL particle size, using gradient gel electrophoresis, and malondialdehyde-modified LDL (MDA-LDL), using an ELISA, and investigated the association between triglyceride (TG) concentrations, LDL size, and MDA-LDL. RESULTS: TG concentrations correlated negatively with the predominant LDL size (r = -0.650) and HDL-C concentration (r = -0.556). The relationship between TG concentration and LDL size, evaluated by measuring MDA-LDL, distinguished subgroups derived from four subfractions of TG concentrations and four distribution ranges of LDL size. These experiments indicated that there is a threshold for oxidation susceptibility at an LDL size of 25.5 nm and a TG concentration of 1500 mg/L. To investigate the relationship between LDL size, MDA-LDL concentration, and other lipids (TGs, HDL-C, apolipoprotein B, and total cholesterol), we evaluated them in control subjects and patients with diabetes mellitus or hypertriglyceridemia. When the size range for normal LDL was postulated to be 25.5 < or = phi (LDL diameter) < 26.5 nm, the MDA-LDL concentration was significantly higher in the subgroups of patients with LDL in the size range 24.5 < or = phi < 25.5 nm compared with patients with normal LDL. This result also suggests that the threshold is at a LDL size of 25.5 nm. CONCLUSION: The threshold for oxidation susceptibility coincided with the point of LDL size separation between the LDL subclass patterns A and B as an atherosclerotic risk.

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AdultAgedDiabetes MellitusElectrophoresis, Polyacrylamide GelEnzyme-Linked Immunosorbent AssayFemaleHumansHypertriglyceridemiaLipoproteins, LDLMaleMalondialdehydeMiddle AgedParticle SizeTriglyceridesJournal ArticleResearch Support, Non-U.S. Gov't

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A Kondo, Y Muranaka, I Ohta, K Notsu, M Manabe, K Kotani, K Saito, M Maekawa, T Kanno. 2001. Relationship between triglyceride concentrations and LDL size evaluated by malondialdehyde-modified LDL.. https://pubmed.ncbi.nlm.nih.gov/11325894/

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