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Xenon does not affect human platelet function in vitro.

L W de Rossi·N A Horn·J H Baumert·K Gutensohn·G Hutschenreuter·R Rossaint
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Abstract

We sought to determine whether xenon affects platelet glycoprotein expression and platelet-related hemostasis in vitro at a clinically relevant concentration. Human whole blood was stimulated with either adenosine diphosphate or the thrombin receptor agonist peptide (TRAP)-6 after incubation with 65% xenon. Halothane at 2 minimum alveolar anesthetic concentration was used as a positive control. Platelet function and activation were evaluated with two-color flow cytometry. The expression of the platelet glycoproteins GPIIb/IIIa, GPIb, and P selectin were detected with fluorochrome-conjugated monoclonal antibodies. In vitro measurement of platelet-related hemostasis under conditions of high shear stress was performed in citrated whole blood with a platelet function analyzer (PFA-100((R))) by using collagen/epinephrine and collagen/adenosine diphosphate cartridges. Xenon did not affect basal or agonist-induced expression of platelet membrane glycoproteins, activation-dependent conformational changes of the GPIIb/IIIa receptor, expression of P selectin, or PFA closure times. In contrast, halothane reduced TRAP-6-induced activation of the GPIIb/IIIa complex. Furthermore, collagen/epinephrine-induced PFA closure time was significantly prolonged. These results demonstrate that xenon does not affect the unstimulated or agonist-induced platelet glycoprotein expression, activation of GPIIb/IIIa, or platelet-related hemostasis.

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Anesthetics, InhalationBlood PlateletsFlow CytometryHalothaneHemostasisHumansIn Vitro TechniquesP-SelectinPlatelet ActivationPlatelet Function TestsPlatelet Glycoprotein GPIIb-IIIa ComplexXenonJournal ArticleResearch Support, Non-U.S. Gov't

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L W de Rossi, N A Horn, J H Baumert, K Gutensohn, G Hutschenreuter, R Rossaint. 2001. Xenon does not affect human platelet function in vitro.. https://doi.org/10.1097/00000539-200109000-00020

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