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PubMed · 11743576

Phenotyping transgenic embryos: a rapid 3-D screening method based on episcopic fluorescence image capturing.

Wolfgang Johann Weninger·Timothy Mohun
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Abstract

We describe a technique suitable for routine three-dimensional (3-D) analysis of mouse embryos that is based on episcopic fluorescence images captured during serial sectioning of wax-embedded specimens. We have used this procedure to describe the cardiac phenotype and associated blood vessels of trisomic 16 (Ts16) and Cited2-null mutant mice, as well as the expression pattern of an Myf5 enhancer/beta-galactosidase transgene. The consistency of the images and their precise alignment are ideally suited for 3-D analysis using video animations, virtual resectioning or commercial 3-D reconstruction software packages. Episcopic fluorescence image capturing (EFIC) provides a simple and powerful tool for analyzing embryo and organ morphology in normal and transgenic embryos.

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Abnormalities, MultipleAnalog-Digital ConversionAnimalsAortaDNA-Binding ProteinsFetal HeartFiber Optic TechnologyFluorometryGenes, ReporterImage Processing, Computer-AssistedImaging, Three-DimensionalLac OperonMiceMice, KnockoutMice, TransgenicMicrotomyMotion PicturesMuscle ProteinsMyogenic Regulatory Factor 5PhenotypePhotomicrographyRecombinant Fusion ProteinsRepressor ProteinsSoftwareStaining and LabelingTissue EmbeddingTrans-ActivatorsTrisomyVena Cava, SuperiorWaxesbeta-GalactosidaseJournal Article

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Wolfgang Johann Weninger, Timothy Mohun. 2001-12-17. Phenotyping transgenic embryos: a rapid 3-D screening method based on episcopic fluorescence image capturing.. https://doi.org/10.1038/ng785

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