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Hepatitis A virus: a test method for virucidal activity.

M H Wolff·J Schmitt·M Rahaus·A König
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Abstract

Hepatitis A virus (HAV) is closely related to the genus enterovirus. HAV is very stable and resistant to acid pH and elevated temperature, as well as to chemicals and environmental influences. Human poliovirus is still one of the model viruses for testing disinfectants but there are discussions about changing to hepatitis A virus. The purpose of this study was to develop a method for using adapted hepatitis A virus to test hand disinfectants. Using HAV strains HM175/24a and FRhK-4 cytopathic effects were visible rarely, and not before 14 days. To verify virus growth in cells a RT-PCR was developed. Two disinfectants tested did not show the required virucidal activity to satisfy current German guidelines.

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2-PropanolAnimalsAnti-Infective Agents, LocalCytopathogenic Effect, ViralDisinfectantsDrug Evaluation, PreclinicalEnzyme-Linked Immunosorbent AssayEthanolGuidelines as TopicHand DisinfectionHepatitis A virusHumansHydrogen-Ion ConcentrationMacaca mulattaReverse Transcriptase Polymerase Chain ReactionTemperatureVirus CultivationJournal ArticleResearch Support, Non-U.S. Gov't

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M H Wolff, J Schmitt, M Rahaus, A König. 2001. Hepatitis A virus: a test method for virucidal activity.. https://doi.org/10.1016/s0195-6701(01)90007-6

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