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Demonstration of four immunoassay formats using the array biosensor.

Kim E Sapsford·Paul T Charles·Charles H Patterson·Frances S Ligler
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Abstract

The ability of a fluorescence-based array biosensor to measure and quantify the binding of an antigen to an immobilized antibody has been demonstrated using the four different immunoassay formats: direct, competitive, displacement, and sandwich. A patterned array of antibodies specific for 2,4,6-trinitrotoluene (TNT) immobilized onto the surface of a planar waveguide and used to measure signals from different antigen concentrations simultaneously. For direct, competitive, and displacement assays, which are one-step assays, measurements were obtained in real time. Dose-response curves were calculated for all four assay formats, demonstrating the array biosensor's ability to quantify the amount of antigen present in solution.

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AntibodiesBiosensing TechniquesEnvironmental PollutantsFluorescent DyesImmunoassayTrinitrotolueneNASA Discipline Life Sciences TechnologiesNon-NASA CenterJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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Kim E Sapsford, Paul T Charles, Charles H Patterson, Frances S Ligler. 2002-03-01. Demonstration of four immunoassay formats using the array biosensor.. https://doi.org/10.1021/ac0157268

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