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PubMed · 11977626

[Cartilage engineering utilizing a porous bioceramic (beta-TCP)-cell complex].

Changyong Wang·Bin Bo·Qiang Zhao·Ximin Guo·Cuimi Duan·Yonghong Wang·Ming Fan·Jianxi Lu
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Abstract

OBJECTIVE: To evaluate the feasibility of growing tissue-engineered cartilage using chondrocytes seeded onto a biodegradable porous bioceramic, the beta-tricalcium phosphate (beta-TCP). METHODS: A porous bioceramic template of beta-TCP was created in the shape of a disc. Chondrocytes isolated from rabbit articular cartilage were seeded on the beta-TCP template and then kept in rotatory cell culture system (RCCS) for 1 week prior to subcutaneous transplantation into athymic mice. The three-dimensional structure was well-maintained 16 weeks after implantation. After 4, 8, 16 weeks, the specimens were harvested and examined macroscopically, histologically and immunohistochemically. RESULTS: Gross morphological and histological analysis of the specimens from the chondrocyte-beta-TCP complex demonstrated new cartilage construction. The overall configuration of the experimental specimens closely resembled the structure of beta-TCP template. CONCLUSION: These findings suggest that porous bioceramic (beta-TCP) is a good "matrix" for chondrocyte, and can be used for cartilage engineering.

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AnimalsCalcium PhosphatesCartilageDNAFemaleGlycosaminoglycansImmunohistochemistryMiceMice, NudeTissue EngineeringEnglish AbstractJournal ArticleResearch Support, Non-U.S. Gov't

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Changyong Wang, Bin Bo, Qiang Zhao, Ximin Guo, Cuimi Duan, Yonghong Wang, Ming Fan, Jianxi Lu. 2002. [Cartilage engineering utilizing a porous bioceramic (beta-TCP)-cell complex].. https://pubmed.ncbi.nlm.nih.gov/11977626/

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