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Monitoring of ethanol during fermentation using a microbial biosensor with enhanced selectivity.

Jan Tkac·Igor Vostiar·Peter Gemeiner·Ernest Sturdik
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Abstract

The present study is concerning the construction of ferricyanide-mediated Gluconobacter oxydans cell ethanol biosensor. The size exclusion effect of a cellulose acetate membrane was used for elimination of glucose interferences during ethanol assays in real samples. A typical response time of the biosensor was 13 s with a high sensitivity of 3.5 microA mM(-1). The microbial biosensor exhibits a very low detection limit of 0.85 microM and a wide linear range from 2 to 270 microM. The operational stability was excellent. During 8.5 h of repetitive ethanol assays, no decrease in the sensor sensitivity was observed. The biosensor was successfully used in the off-line monitoring of ethanol fermentation with a good agreement with HPLC measurements (R(2)=0.998).

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Biosensing TechniquesEthanolFermentationGluconobacter oxydansSensitivity and SpecificityJournal ArticleResearch Support, Non-U.S. Gov't

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Jan Tkac, Igor Vostiar, Peter Gemeiner, Ernest Sturdik. 2002-05-15. Monitoring of ethanol during fermentation using a microbial biosensor with enhanced selectivity.. https://doi.org/10.1016/s1567-5394(02)00054-3

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