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PubMed · 12018026

Quantitative determination for cytotoxic Friedo-nor-oleanane derivatives from five morphological types of Maytenus ilicifolia (Celastraceae) by reverse-phase high-performance liquid chromatography.

Waldemar Buffa Filho·Joaquim Corsino·da Silva Vanderlan Bolzani·Maysa Furlan·Ana Maria S Pereira·Suzelei Castro França
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Abstract

Five different morphological types of Maytenus ilicifolia of the same age and harvested under the same conditions showed distinct accumulations of some friedo-nor-oleananes. A rapid, sensitive and reliable reverse-phase HPLC method (employing an external standard) was used for the determination of the cytotoxic triterpenoids, 20 alpha-hydroxymaytenin, 22 beta-hydroxymaytenin, maytenin, celastrol and pristimerin in each of the five types. Well resolved peaks with good detection response and linearity in the range 1.0-100 micrograms/mL were obtained.

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Antineoplastic Agents, PhytogenicBrazilChromatography, High Pressure LiquidMaytenusMolecular StructureOleanolic AcidPlant BarkPlant LeavesPlant RootsReference StandardsSensitivity and SpecificityTriterpenesJournal ArticleResearch Support, Non-U.S. Gov't

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Waldemar Buffa Filho, Joaquim Corsino, da Silva Vanderlan Bolzani, Maysa Furlan, Ana Maria S Pereira, Suzelei Castro França. Quantitative determination for cytotoxic Friedo-nor-oleanane derivatives from five morphological types of Maytenus ilicifolia (Celastraceae) by reverse-phase high-performance liquid chromatography.. https://doi.org/10.1002/pca.626

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