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PubMed · 12051737

Functional interaction between Brn-3a and Src-1 co-activates Brn-3a-mediated transactivation.

Jonathan H Dennis·Vishwanie Budhram-Mahadeo·David S Latchman
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Abstract

The Brn-3a POU domain transcription factor is able to regulate the transcription of promoters containing a Brn-3 response element via its POU domain. In addition, the POU domain of Brn-3a has been shown to functionally interact with the estrogen receptor and regulate transcription from estrogen responsive promoters. The steroid receptor coactivator, Src-1, enhances transcription with a variety of steroid receptors. Here we describe a functional interaction between Brn-3a and Src-1. In glutathione S-transferase pull-down assays Src-1 was shown to specifically interact with Brn-3 proteins. Moreover, Src-1 co-immunoprecipitated from intact cells with Brn-3a. The transactivation potential of the Brn-3a/Src-1 complex was tested on both the Brn-3 responsive SNAP-25 promoter and the estrogen responsive vitellogenin promoter, in each of two different cell lines, the neuronal ND7 cell line, and the kidney BHK21 cell line. Src-1 consistently and strongly potentiated the activation of Brn-3a on the SNAP promoter construct in both the ND7 and BHK21 cell lines. The vitellogenin promoter construct, however, was only weakly activated by the Brn-3/Src-1 complex in the ND7 cells and there was even less effect on this promoter in the BHK21 cells. These results suggest a functional role for Src-1 in enhancing Brn-3a mediated transactivation, seemingly independent of nuclear hormone receptors, thus broadening the transcriptional repertoire of both Brn-3a and Src-1.

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AnimalsCell LineChromatography, AffinityCricetinaeDNA-Binding ProteinsDose-Response Relationship, DrugGlutathione TransferaseHistone AcetyltransferasesKidneyMacromolecular SubstancesMembrane ProteinsMiceNerve Tissue ProteinsNeuronsNuclear Receptor Coactivator 1Precipitin TestsPromoter Regions, GeneticProtein BindingRatsRecombinant Fusion ProteinsSynaptosomal-Associated Protein 25Trans-ActivatorsTranscription Factor Brn-3Transcription Factor Brn-3ATranscription FactorsTranscriptional ActivationTransfectionVitellogeninsJournal Article

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Jonathan H Dennis, Vishwanie Budhram-Mahadeo, David S Latchman. 2002-06-07. Functional interaction between Brn-3a and Src-1 co-activates Brn-3a-mediated transactivation.. https://doi.org/10.1016/s0006-291x(02)00500-4

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