Efficient embedding technique for preparing small specimens for stereological volume estimation: zebrafish larvae.

A J Hill; C V Howard; A R Cossins

doi:10.1046/j.1365-2818.2002.01025.x

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Efficient embedding technique for preparing small specimens for stereological volume estimation: zebrafish larvae.

A J Hill·C V Howard·A R Cossins

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Abstract

Stereological sampling regimes, in particular volume and number estimation, often require systematic uniformly random sections throughout a specimen. A method has been developed to increase the efficiency of preparing fish larvae for sectioning prior to histological or stereological analysis. Embedding a group of larvae in a resin block using this technique greatly reduces the quantity of sections produced and allows easy assessment of sample groups. Saving time in this way therefore makes stereology a more viable research tool.

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Animals Histological Techniques Larva Plastic Embedding Specimen Handling Tissue Embedding Zebrafish Journal Article

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A J Hill, C V Howard, A R Cossins. 2002. Efficient embedding technique for preparing small specimens for stereological volume estimation: zebrafish larvae.. https://doi.org/10.1046/j.1365-2818.2002.01025.x

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