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PubMed · 12080729

[Sample preparation for electron microscope with tissues fixed with formalin and embedded in paraffin].

J Jing
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Abstract

A method of sample preparation for electron microscope is introduced. After paraffin-embedded tissues, fresh and fixed on time, were deparaffined, the tissue were fixed in 4% glutaraldehyde for 24 hours, in 1% osmic acid for 1-2 hours, and dehydrated with acetone and embedded in Epon812; then they were cut into ultrathin sections. By this method, better ultrastructure of paraffin-embedded tissues was obtained, and the method could satisfy the demand for observation under electron microscope. In addition, some important proceedings were also discussed in the paper.

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FormaldehydeHumansIntestinal NeoplasmsParaffin EmbeddingSpecimen HandlingTissue FixationEnglish AbstractJournal Article

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J Jing. 1999. [Sample preparation for electron microscope with tissues fixed with formalin and embedded in paraffin].. https://pubmed.ncbi.nlm.nih.gov/12080729/

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