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Poly(ethylene glycol) hydrogel microstructures encapsulating living cells.

Won-Gun Koh·Alexander Revzin·Michael V Pishko
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Abstract

We present an easy and effective method for the encapsulation of cells inside PEG-based hydrogel microstructures fabricated using photolithography. High-density arrays of three-dimensional microstructures were created on substrates using this method. Mammalian cells were encapsulated in cylindrical hydrogel microstructures of 600 and 50 micrometers in diameter or in cubic hydrogel structures in microfluidic channels. Reducing lateral dimension of the individual hydrogel microstructure to 50 micrometers allowed us to isolate 1-3 cells per microstructure. Viability assays demonstrated that cells remained viable inside these hydrogels after encapsulation for up to 7 days.

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AnimalsBiocompatible MaterialsBiosensing TechniquesBiotechnologyCell LineCell SurvivalCells, CulturedFibroblastsHydrogelsMicePolyethylene GlycolsUltraviolet RaysNASA Discipline Life Sciences TechnologiesNon-NASA CenterJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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Won-Gun Koh, Alexander Revzin, Michael V Pishko. 2002-04-02. Poly(ethylene glycol) hydrogel microstructures encapsulating living cells.. https://doi.org/10.1021/la0115740

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