Peptide dendrimers based on polyproline helices.

Laia Crespo; Glòria Sanclimens; Beatriz Montaner; Ricardo Pérez-Tomás; Miriam Royo; Miquel Pons; Fernando Albericio; Ernest Giralt

doi:10.1021/ja020364m

PubMed · 12137542

Peptide dendrimers based on polyproline helices.

Laia Crespo·Glòria Sanclimens·Beatriz Montaner·Ricardo Pérez-Tomás·Miriam Royo·Miquel Pons·Fernando Albericio·Ernest Giralt

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Abstract

We present a new family of peptide dendrimers based on polyproline helices and cis-4-amino-L-proline as a branching unit. Dendrimers were synthesized by a convergent solid-phase peptide synthesis approach. The conformational transition between polyproline type I helix and polyproline type II helix was observed by circular dichroism in branched polyproline building blocks with more than 14 proline residues and in the resulting dendrimers. Both linear and dendritic polyprolines were found to be actively internalized by rat kidney cells. Preliminary results show that the antibiotic ciprofloxacin form complexes with branched polyproline chains in 99.5% propanol.

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Animals Anti-Infective Agents Cell Line Ciprofloxacin Circular Dichroism Kidney Microscopy, Fluorescence Peptides Protein Structure, Secondary Rats Journal Article Research Support, Non-U.S. Gov't

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Laia Crespo, Glòria Sanclimens, Beatriz Montaner, Ricardo Pérez-Tomás, Miriam Royo, Miquel Pons, Fernando Albericio, Ernest Giralt. 2002-07-31. Peptide dendrimers based on polyproline helices.. https://doi.org/10.1021/ja020364m

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