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PubMed · 12450529

Simultaneous solid-phase extraction combined with liquid chromatography with ultraviolet absorbance detection for the determination of remifentanil and its metabolite in dog plasma.

M Kabbaj·F Varin
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Abstract

To establish pharmacokinetic/pharmacodynamic relationships, a selective and specific high-performance liquid chromatographic method was developed for the quantitation of remifentanil and its metabolite in dog plasma. The assay involves a solid-phase extraction and a reversed-phase chromatographic separation with ultraviolet detection (lambda=210 nm). The calibration curves are linear in the range of 7.89-1500 ng ml(-1). Intra-day assay variability is less than 7% for all standards evaluated. Good recovery, linearity, accuracy, and precision were achieved with the assay that proved readily applicable to pharmacokinetic studies in dogs.

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Analgesics, OpioidAnimalsChromatography, High Pressure LiquidDogsPiperidinesRemifentanilReproducibility of ResultsSensitivity and SpecificitySpectrophotometry, UltravioletJournal ArticleResearch Support, Non-U.S. Gov't

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M Kabbaj, F Varin. 2003-01-05. Simultaneous solid-phase extraction combined with liquid chromatography with ultraviolet absorbance detection for the determination of remifentanil and its metabolite in dog plasma.. https://doi.org/10.1016/s1570-0232(02)00524-x

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