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A new method for fish leucocyte counting and partial differentiation by flow cytometry.

Takamasa Inoue·Tadaaki Moritomo·Yumi Tamura·Sachiko Mamiya·Hiroshi Fujino·Teruyuki Nakanishi
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Abstract

A simple and rapid method for analysis of fish blood cells is presented. Carp (Cyprinus carpio) blood was diluted 200 times with Hanks' solution containing 1 microg/ml of DiOC6(3) which is a fluorescent, lipophilic dye. After staining for 10 min, the blood cells were measured by a flow cytometer (FACS). Several blood cell populations were identified by different FL-1 (green fluorescence), FSC (forward scatter), and SSC (side scatter) properties. FL-1 v. SSC or FSC v. SSC dot-plot of stained blood cells displayed five separate cell populations: erythrocytes: a mixture of thrombocytes plus lymphocytes; monocytes; neutrophils; and basophils. The number of each type of blood cell counted by the FACS was in good agreement with those counted microscopically.

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AnimalsBlood Cell CountCarpsCell SeparationErythrocyte CountFlow CytometryFluorescent DyesLeukocyte CountLeukocytesLymphocyte CountPlatelet CountStaining and LabelingJournal Article

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Takamasa Inoue, Tadaaki Moritomo, Yumi Tamura, Sachiko Mamiya, Hiroshi Fujino, Teruyuki Nakanishi. 2002. A new method for fish leucocyte counting and partial differentiation by flow cytometry.. https://doi.org/10.1006/fsim.2002.0413

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