PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 12485342

Bacteriophage isolation from human saliva.

Gilad Bachrach·M Leizerovici-Zigmond·A Zlotkin·R Naor·D Steinberg
View original source ↗Export metadata ↓

Abstract

AIMS: To detect bacteriophages for Gram-positive oral pathogens in human saliva. METHODS AND RESULTS: Saliva samples from 31 donors were screened for the presence of bacteriophages for Streptococcus sobrinus, Streptococcus mutans, Streptococcus salivarius, Actinomyces viscosus and Enterococcus faecalis. Bacteriophages for Enterococcus faecalis were found in seven samples. Enterococcus faecalis phages were still present in saliva re-collected from one donor one month, and one year after initial saliva collection. CONCLUSIONS: The presence and stability of the Enterococcus faecalis bacteriophages in human saliva suggests a possible role of these bacteriophages in the oral ecosystem. SIGNIFICANCE AND IMPACT OF THE STUDY: Phage therapy as a way to control oral bacteria might be considered.

Explore related subjects

Actinomyces viscosusBacteriophage lambdaBacteriophagesBase SequenceEnterococcus faecalisGram-Positive BacteriaHumansMicroscopy, ElectronMolecular Sequence DataRNA, Ribosomal, 16SSalivaStreptococcusJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Gilad Bachrach, M Leizerovici-Zigmond, A Zlotkin, R Naor, D Steinberg. 2003. Bacteriophage isolation from human saliva.. https://doi.org/10.1046/j.1472-765x.2003.01262.x

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2006

An in vitro quantitative antibacterial analysis of amalgam and composite resins.

OBJECTIVES: Antibacterial properties of restorative dental materials such as amalgam and composite resins may improve the restorative treatment outcome. This study evaluates the antibacterial properties of three composite resins: Z250, Tetric Ceram, P60 and a dental amalgam in vitro. METHODS: Streptococcus mutans and Actinomyces viscosus served as test microorganisms. Three quantitative microtiter spectrophotometric assays were used to evaluate the effect of the restorative materials on: (i) early-stage biofilm using a direct contact test (DCT); (ii) planktonic bacterial growth; (iii) bacterial growth in the materials' elute. For comparison purposes, agar diffusion test (ADT) was also performed. RESULTS: The effect of the composite resins on bacterial growth was minimal and limited to a few days only. One-week-aged composites promoted growth of S. mutans and A. viscosus. The antibacterial properties in direct contact were more potent than in planktonic bacterial growth. Amalgam showed complete inhibition of both bacteria in all phases, and the effect lasted for at least 1 week. The materials' elute had no effect on both bacterial growth with the exception of complete inhibition of S. mutans in amalgam. The later results correlated with the ADT. CONCLUSIONS: The present findings demonstrate potent and lasting antibacterial properties of amalgam, which are lacking in composite resins. This may explain the clinical observation of biofilm accumulated more on composites compared to amalgams. It follows that the assessment of antibacterial properties of poorly-soluble materials has to employ more than one assay.

Actinomyces viscosus↗
PubMed2006

The antibacterial effects of zinc ion migration from zinc-based glass polyalkenoate cements.

Zinc-based glass polyalkenoate cements have been synthesised and their potential use in orthopaedic applications investigated. Zinc ions were released from the materials in a rapid burst over the first 24 h after synthesis, with the release rate falling below detectable levels after 7 days. Cement-implanted bone samples were prepared and the released zinc was shown, using energy dispersive X-ray analysis, to penetrate from the cement into the adjacent bone by up to 40 microm. Finally, the cements exhibited antibacterial activity against Streptococcus mutans and Actinomyces viscosus that reflected the pattern of zinc release, with the inhibition of growth greatest shortly after cement synthesis and little or no inhibition measureable after 30 days.

Actinomyces viscosus↗
PubMed2006

In vitro binding interactions of oral bacteria with immobilized fructosyltransferase.

AIMS: The objective of the present study was to explore the role of immobilized fructosyltransferase (FTF) in adhesion process. METHODS AND RESULTS: We investigated real-time biospecific interactions between several types of oral bacteria and recombinant FTF immobilized on a biosensor chip, using surface plasmon resonance technology. Streptococcus mutans, Streptococcus sobrinus and Actinomyces viscosus demonstrated significant binding to FTF. Actinomyces viscosus had a greater binding to FTF, with 373 Resonance Units (RU), than the other tested bacteria. The binding level to FTF of Strep. sobrinus was 320 RU, whereas Strep. mutans and Streptococcus salivarious show binding of 296 and 245 RU, respectively. The binding sensograms displayed different profiles for the tested bacteria at various cell density, suggesting a different affinity to immobilized FTF. CONCLUSIONS: The results from this study suggest that FTF may influence bacterial adherence and colonization of the dental biofilm. SIGNIFICANCE AND IMPACT OF THE STUDY: The biomolecular interaction analysis enables real-time monitoring of the interaction between adhesions of intact bacteria and their ligands, which might be crucial in the initial phase of biofilm development in vivo.

Actinomyces viscosus↗