Application of co-eluting structural analog internal standards for expanded linear dynamic range in liquid chromatography/electrospray mass spectrometry.

Guoen Shi

doi:10.1002/rcm.897

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Application of co-eluting structural analog internal standards for expanded linear dynamic range in liquid chromatography/electrospray mass spectrometry.

Guoen Shi

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Abstract

Non-linear standard calibration curves occur frequently in liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS), necessitating the use of non-linear regression curve fitting or a reduction in assay dynamic range. Non-linearity can be minimized by the use of stable isotope internal standards, if the cause of curve bending is ionization saturation. Unfortunately, stable isotope internal standards can be difficult or expensive to synthesize. Structural analog internal standards, if sufficiently close to analytes both in chemical structures and HPLC retention times, may mimic the effect of stable isotope internal standards. Tentative experimental evidence supporting this concept is presented in this paper.

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Animals Calibration Chromatography, High Pressure Liquid Dogs Isotopes Reference Standards Reproducibility of Results Spectrometry, Mass, Electrospray Ionization Journal Article

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Guoen Shi. 2003. Application of co-eluting structural analog internal standards for expanded linear dynamic range in liquid chromatography/electrospray mass spectrometry.. https://doi.org/10.1002/rcm.897

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