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PubMed · 12567867

[Quality control methods for recombinant human endostatin].

Yong-Hong Li·Jun-zhi Wang·Chun-mei Han·Yi Zhang·Chun-ming Rao
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Abstract

AIM: To establish the quality control methods for recombinant human endostatin. METHODS: Biological activity was determined by endothelial cell migration assays. Peptide mapping was tested by trypsin digestion and RP-HPLC. Purity was determined by non-reduced SDS-PAGE and RP-HPLC. Other tests including molecular weight, isoelectrical point, etc. were done according to the National Requirements for Biological Products (2000). RESULTS: The method of bioassay was established and used for determining activity of endostatin. Specific activity of the three batchs of drug substance was 1.45 x 10(6), 1.57 x 10(6) and 2.73 x 10(6) u.mg-1 proteins. Peptide mappings of the three batches of drug substance were completely identified. Both purity results of the products tested by SDS-PAGE and RP-HPLC were more than 99%. CONCLUSION: The established methods can effectively control the quality of recombinant human endostatin.

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Cell MovementCells, CulturedEndostatinsEndothelium, VascularHumansQuality ControlRecombinant ProteinsTechnology, PharmaceuticalEnglish AbstractJournal Article

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Yong-Hong Li, Jun-zhi Wang, Chun-mei Han, Yi Zhang, Chun-ming Rao. 2002. [Quality control methods for recombinant human endostatin].. https://pubmed.ncbi.nlm.nih.gov/12567867/

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