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Development, characterization, and validation of a sensitive primate-specific quantification assay for forensic analysis.

John C Fox·Christopher A Cave·James W Schumm
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Abstract

Accurate human-specific DNA quantification is essential for forensic casework analysis. In this work, we describe a microplate-based quantification assay that utilizes the PCR amplification of human-specific TH01 primers. This method enables the reliable quantification of human DNA samples from 0.2 to 40 ng, even in mixtures with nonhuman DNA. Analysis of samples can be semi-automated using 96-well microplates and a spreadsheet-based concentration calculator for high-throughput demands. We have used this quantification method with more than 15,000 forensic samples.

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AnimalsDNAFluorometryForensic MedicineHumansMacaca mulattaMicrochemistryPolymerase Chain ReactionPrimatesQuality ControlRabbitsReproducibility of ResultsSensitivity and SpecificitySoftwareSpecies SpecificityComparative StudyEvaluation StudyJournal ArticleValidation Study

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John C Fox, Christopher A Cave, James W Schumm. 2003. Development, characterization, and validation of a sensitive primate-specific quantification assay for forensic analysis.. https://doi.org/10.2144/03342rr02

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