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PubMed · 1270386

A screening method for protein characterization and differentiation.

G F Pinto·V L Costa-Carvalho·E R Souza·J S Araújo Neto
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Abstract

A circular paper chromatographic method was developed for the separation of the amino acids in proteins into 7 subgroups. Butanol-acetic acid water (4+1+1) was used as the developing solvent. Eluted ninhydrin-stained aminograms gave rise to graphic profiles or numerical indexes based on absorbance percentages. The profiles can be used to compare protein-containing samples. Twenty different samples were studied through 190 comparisons of graphic profiles and coefficients of correlation, with only 4% misleading results. The method showed excellent reproducibility for the identification or differentiation of proteins and has the advantage of being performed with low-priced apparatus and reagents.

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Amino AcidsChromatography, PaperFood AnalysisMethodsProtein HydrolysatesProteinsSolventsJournal Article

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G F Pinto, V L Costa-Carvalho, E R Souza, J S Araújo Neto. 1976. A screening method for protein characterization and differentiation.. https://pubmed.ncbi.nlm.nih.gov/1270386/

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