PubMed · 12738536
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
Wilfried Feichtinger. 2003. Cryopreservation techniques.. https://doi.org/10.1016/s0015-0282(02)04954-3
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
Marine diplonemids (Diplonemidae and Hemistasiidae), once considered difficult to culture, can now be introduced into stable axenic cultures and grown to high densities. This chapter provides detailed protocols on establishing clonal cultures from environmental cells, their axenization and routine maintenance, as well as instructions on mid- and large-scale cultivation, estimation of cell density, and cryopreservation of selected species.
REVIEW: The outcome of freeze-thaw cycles with pronuclear stage oocytes or embryos, derived from collecting cycles stimulated with gonadotropin-releasing hormone (GnRH)-antagonists' protocols, was reviewed. CONCLUSION: The viability of cryopreserved pronuclear stage oocytes and embryos, the quality of transferred embryos and the pregnancy rates of the freeze-thaw cycles seem to be satisfactory regardless of the type and dose of GnRH-antagonist.
Transfer of donated frozen embryos of various quality to recipients resulted in a delivered pregnancy rate of 36.8% per transfer. Hopefully these data will encourage other in vitro fertilization programs to set up similar programs.