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PubMed · 12800930

Coupling solid-phase microextraction and high-performance liquid chromatography for direct and sensitive determination of halogenated fungicides in wine.

S Millán·M C Sampedro·N Unceta·M A Goicolea·E Rodríguez·R J Barrio
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Abstract

A solid-phase microextraction (SPME) method coupled to high-performance liquid chromatography with diode array detection (HPLC-DAD) for the analysis of six organochlorine fungicides (nuarimol, triadimenol, triadimefon, folpet, vinclozolin and penconazole) in wine was developed. For this purpose, polydimethylsiloxane-divinylbenzene-coated fibers were utilized and all factors affecting throughput, precision, and accuracy of the SPME method were investigated and optimized. These factors include: matrix influence, extraction and desorption time, percentage of ethanol, pH, salt effect and desorption mode. The performed analytical procedure showed detectability ranging from 4 to 27 microg l(-1) and precision from 2.4 to 14.2% (as intra-day relative standard deviation, RSD) and 4.7-25.7% (as inter-day RSD) depending on the fungicide. The results demonstrate the suitability of the SPME-HPLC-DAD method to analyze these organochlorine fungicides in red wine.

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Chromatography, High Pressure LiquidFungicides, IndustrialHalogensReproducibility of ResultsSensitivity and SpecificityWineJournal ArticleResearch Support, Non-U.S. Gov't

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S Millán, M C Sampedro, N Unceta, M A Goicolea, E Rodríguez, R J Barrio. 2003-05-02. Coupling solid-phase microextraction and high-performance liquid chromatography for direct and sensitive determination of halogenated fungicides in wine.. https://doi.org/10.1016/s0021-9673(03)00521-1

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