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Towards saliva-based screening for sleeping sickness?

V Lejon·J Kwete·P Büscher
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Abstract

A pilot study was carried out on the detection of trypanosome-specific antibodies in saliva for diagnosis of sleeping sickness. All twenty-three saliva samples of parasitologically confirmed Trypanosoma brucei gambiense patients tested positive in an indirect enzyme-linked immunosorbent assay, whereas all 14 saliva samples of a negative control group remained negative. Trypanosome-specific antibody levels in patient saliva correlated with antibody levels in serum, but were about 250-fold lower. Eight of 23 undiluted saliva samples of sleeping sickness patients tested positive in CATT/T. b. gambiense and two of 23 in LATEX/T. b. gambiense. All fourteen saliva samples of the negative control group were also positive in CATT/T. b. gambiense, as were four of 14 in LATEX/T. b. gambiense. CATT and LATEX were thus inappropriate for antibody detection in saliva. These results indicate that trypanosome-specific antibody detection in saliva is possible. This could lead to the development of a simple, non-invasive, reliable saliva field test for diagnosis of sleeping sickness.

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AnimalsAntibodies, ProtozoanEnzyme-Linked Immunosorbent AssayFluorescent Antibody TechniqueHumansLatex Fixation TestsPilot ProjectsReagent Kits, DiagnosticReproducibility of ResultsSalivaSensitivity and SpecificityTrypanosoma brucei gambienseTrypanosomiasis, AfricanJournal Article

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V Lejon, J Kwete, P Büscher. 2003. Towards saliva-based screening for sleeping sickness?. https://doi.org/10.1046/j.1365-3156.2003.01077.x

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