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DNA: a programmable force sensor.

Christian Albrecht·Kerstin Blank·Mio Lalic-Mülthaler·Siegfried Hirler·Thao Mai·Ilka Gilbert·Susanne Schiffmann·Tom Bayer·Hauke Clausen-Schaumann·Hermann E Gaub
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Abstract

Direct quantification of biomolecular interaction by single-molecule force spectroscopy has evolved into a powerful tool for materials and life sciences. We introduce an approach in which the unbinding forces required to break intermolecular bonds are measured in a differential format by comparison with a known reference bond (here, a short DNA duplex). In addition to a marked increase in sensitivity and force resolution, which enabled us to resolve single-base pair mismatches, this concept allows for highly specific parallel assays. This option was exploited to overcome cross-reactions of antibodies in a protein biochip application.

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AnimalsAntibodiesBase Pair MismatchBiosensing TechniquesCarbocyaninesCross ReactionsDNADimethylpolysiloxanesFluorescenceFluorescent DyesGlassHumansImmunoassayInterleukin-5MiceMicroscopy, Atomic ForceNucleic Acid ConformationNucleic Acid HybridizationOligodeoxyribonucleotidesOligonucleotide Array Sequence AnalysisProtein Array AnalysisProtein BindingSiliconesTemperatureThermodynamicsJournal ArticleResearch Support, Non-U.S. Gov't

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Christian Albrecht, Kerstin Blank, Mio Lalic-Mülthaler, Siegfried Hirler, Thao Mai, Ilka Gilbert, Susanne Schiffmann, Tom Bayer, Hauke Clausen-Schaumann, Hermann E Gaub. 2003-07-18. DNA: a programmable force sensor.. https://doi.org/10.1126/science.1084713

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