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Protein-DNA interaction mapping using genomic tiling path microarrays in Drosophila.

Ling V Sun·Liang Chen·Frauke Greil·Nicolas Negre·Tong-Ruei Li·Giacomo Cavalli·Hongyu Zhao·Bas Van Steensel·Kevin P White
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Abstract

We demonstrate the use of a chromosomal walk (or "tiling path") printed as DNA microarrays for mapping protein-DNA interactions across large regions of contiguous genomic DNA in Drosophila melanogaster. Microarrays were constructed with genomic DNA fragments 430-920 bp in length, covering 2.9 million base pairs of the Adh-cactus region of chromosome 2 and 85,000 base pairs of the 82F region of chromosome 3. We performed DNA localization mapping for the heterochromatin protein HP1 and for the sequence-specific GAGA transcription factor, producing a comprehensive, high-resolution map of in vivo protein-DNA interactions throughout these regions of the Drosophila genome.

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Amino Acid MotifsAnimalsChromatinDNADNA-Binding ProteinsDrosophilaOligonucleotide Array Sequence AnalysisPrecipitin TestsProteinsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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Ling V Sun, Liang Chen, Frauke Greil, Nicolas Negre, Tong-Ruei Li, Giacomo Cavalli, Hongyu Zhao, Bas Van Steensel, Kevin P White. 2003-07-22. Protein-DNA interaction mapping using genomic tiling path microarrays in Drosophila.. https://doi.org/10.1073/pnas.1533393100

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