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PubMed · 12906079

Optical heterodyne surface-plasmon resonance biosensor.

Wen-Chuan Kuo·Chien Chou·Hsieh-Ting Wu
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Abstract

A novel optical heterodyne surface-plasmon resonance (SPR) biosensor with a Zeeman laser is proposed. Two surface plasma waves are excited by two correlated p-polarized waves in a SPR device of the Kretschmann configuration. Two reflected p waves are optically heterodyned such that the magnitude of the heterodyned signal is proportional to the multiplication of two attenuated reflected p waves. Then the detection sensitivity and the dynamic range based on this amplitude-sensitive method are enhanced. In the experiment, the kinetics between mouse immunoglobulin G (IgG) and rabbit antimouse IgG is obtained from sensograms of various concentrations of antimouse IgG. A detection sensitivity of 0.2 nM was achieved. In addition, a concentration of 5 ng/ml of protein G interacting with mouse IgG was measured successfully.

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AnimalsAntibodiesBiosensing TechniquesImmunoglobulin GKineticsMiceModels, TheoreticalOsmolar ConcentrationRabbitsSurface Plasmon ResonanceJournal Article

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Wen-Chuan Kuo, Chien Chou, Hsieh-Ting Wu. 2003-08-01. Optical heterodyne surface-plasmon resonance biosensor.. https://doi.org/10.1364/ol.28.001329

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