Bone structure and mineralization demonstrated using synchrotron radiation computed tomography (SR-CT) in animal models: preliminary findings.

Masako Ito; Sadakazu Ejiri; Hiroshi Jinnai; Jun Kono; Satoshi Ikeda; Akifumi Nishida; Kentaro Uesugi; Naoto Yagi; Mikako Tanaka; Kuniaki Hayashi

doi:10.1007/s00774-003-0422-x

PubMed · 12928829

Bone structure and mineralization demonstrated using synchrotron radiation computed tomography (SR-CT) in animal models: preliminary findings.

Masako Ito·Sadakazu Ejiri·Hiroshi Jinnai·Jun Kono·Satoshi Ikeda·Akifumi Nishida·Kentaro Uesugi·Naoto Yagi·Mikako Tanaka·Kuniaki Hayashi

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Abstract

We investigated the ability of synchrotron radiation computed tomography (SR-CT) to demonstrate trabecular microstructure, detail of trabecular surfaces, and mineralization of bones. Eight rat vertebrae, six rat tibiae, and eight minipig vertebrae were scanned using SR-CT at the synchrotron radiation facility Super Photon ring-8GeV (SPring-8). Images obtained using conventional micro-CT, scanning electron microscopy (SEM), and contact microradiography (CMR) were compared with the SR-CT images. SR-CT showed high image quality without visible partial volume effect. Three-dimensional SR-CT revealed shallow concavities in the bone surface, which were considered to correspond to osteoclastic resorption areas, as well as the connectivity, anisotropy, and shape (rod- or platelike) of trabeculae. Two-dimensional SR-CT showed different density along the surface of the trabecular bone, indicating the degree of bone mineralization. In conclusion, SR-CT seems to be a useful tool for delineating trabecular surfaces, evaluating bone mineralization, and revealing precise trabecular structure.

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Animals Bone and Bones Calcification, Physiologic Female Models, Animal Rats Spine Synchrotrons Tibia Tomography, X-Ray Computed Journal Article Research Support, Non-U.S. Gov't

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Masako Ito, Sadakazu Ejiri, Hiroshi Jinnai, Jun Kono, Satoshi Ikeda, Akifumi Nishida, Kentaro Uesugi, Naoto Yagi, Mikako Tanaka, Kuniaki Hayashi. 2003. Bone structure and mineralization demonstrated using synchrotron radiation computed tomography (SR-CT) in animal models: preliminary findings.. https://doi.org/10.1007/s00774-003-0422-x

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