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Optic nerve alterations in apolipoprotein E deficient mice.

E Lopez-Sanchez·E Frances-Muñoz·A Diez-Juan·V Andres·J L Menezo·M D Pinazo-Duran
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Abstract

PURPOSE: To study the morphologic characteristics of the optic nerve (ON) by using an experimental model of knockout mice for expression of the ApoE gene. METHODS: Eyeballs with the retrobulbar ON attached were obtained from 24-week-old mice. Using morphologic and morphometric techniques and light and transmission electron microscopy, the ON characteristics were determined in three groups of mice: 1) wild type mice as the controls (CG; n = 15), 2) knockout mice for the ApoE gene (ApoE-G; n = 15), and 3) knockout mice for the ApoE gene that were fed a cholesterol-supplemented diet (ApoED-G; n = 15). RESULTS: The ON cross-sectional area was significantly higher in the ApoE-G than in the CG (p < 0.001) mice, whereas no significant changes were noticed between the ApoE-G and ApoED-G mice. Significant differences were noticed between those groups regarding the myelination index. Higher density of intra-axonal degeneration and myelin sheath alterations were found in both ApoE groups in respect to the CG. CONCLUSIONS: These results suggest that ApoE knockout mice have changes in ON morphology and myelination.

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AnimalsApolipoproteins EAxonsCholesterolCholesterol, DietaryDiet, AtherogenicFemaleMaleMiceMice, Inbred C57BLMice, KnockoutMicroscopy, ElectronMyelin SheathOptic NerveOptic Nerve DiseasesComparative StudyJournal Article

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E Lopez-Sanchez, E Frances-Muñoz, A Diez-Juan, V Andres, J L Menezo, M D Pinazo-Duran. 2003. Optic nerve alterations in apolipoprotein E deficient mice.. https://doi.org/10.1177/112067210301300609

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