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PubMed · 12949952

Fluorescence hysteroscopy using 5-aminolevulinic: a descriptive study.

Pius Wyss·Andrea Degen·Rosmarie Caduff·Rene Hornung·Urs Haller·Mathias Fehr
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Abstract

BACKGROUND AND OBJECTIVES: To assess the feasibility of fluorescence hysteroscopy following topical ALA application and to describe the optical appearance of normal and neoplastic endometrium. STUDY DESIGN/MATERIALS AND METHODS: Fifty-four patients scheduled for routine hysteroscopy and D&C were assigned to the study group using topically applied ALA. Two milliliters of a 2% 5-ALA-solution at pH = 4.0 (ASAT AG/Zug, Switzerland) was administrated into the uterine cavity 4 hours before fluorescence hysteroscopy, using a STORZ-D-Light (Tuttlingen, Germany) system. Hysteroscopic findings were compared to the histological diagnosis of hematoxylin & eosin (H&E) stained sections. In the study group, 60 histological specimens were taken (47 materials of curettage and 13 targeted biopsies). The percentage of fluorescence positive patients was calculated. RESULTS: The rate of fluorescent endometrial samples was low in atrophic endometrium 1/16 (6.2%). Endometrial carcinoma were diagnosed in two patients. Both cases exhibited selective and intense PpIX-mediated red fluorescence. Fluorescence hysteroscopy could not differentiate between hyperplastic areas with or without atypia. Minimal uterine cramps or discomfort following ALA instillation were complained by 30 (56%) patients, no pain was indicated by 24 patients (44%). CONCLUSIONS: Fluorescence hysteroscopy following intrauterine application of ALA is feasible. Cancerous, hyperplastic, and secretory endometrial tissue showed high red fluorescence.

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Administration, TopicalAdultAgedAged, 80 and overAminolevulinic AcidEndometrial HyperplasiaEndometrial NeoplasmsFeasibility StudiesFemaleFluorescenceHumansHysteroscopyMiddle AgedPhotosensitizing AgentsPilot ProjectsJournal ArticleResearch Support, Non-U.S. Gov't

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Pius Wyss, Andrea Degen, Rosmarie Caduff, Rene Hornung, Urs Haller, Mathias Fehr. 2003. Fluorescence hysteroscopy using 5-aminolevulinic: a descriptive study.. https://doi.org/10.1002/lsm.10210

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