Protamine kinase phosphorylates eukaryotic protein synthesis initiation factor 4E.

G D Amick; Z Damuni

doi:10.1016/0006-291x(92)90499-b

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Protamine kinase phosphorylates eukaryotic protein synthesis initiation factor 4E.

G D Amick·Z Damuni

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Abstract

Up to 1 mol of phosphoryl groups was incorporated per mol of eukaryotic protein synthesis initiation factor (eIF) 4E following incubation of purified preparations of this factor with purified preparations of a protamine kinase from bovine kidney cytosol. By contrast, purified preparations of two forms of mitogen-activated protein kinase, casein kinase II and two forms of a distinct autophosphorylation-activated protein kinase exhibited little activity, if any, with eIF-4E. Together with previous observations, the results indicate that the protamine kinase could contribute to the insulin-stimulated phosphorylation of eIF-4E.

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Animals Cattle Eukaryotic Initiation Factor-4E Kidney Peptide Initiation Factors Phosphorylation Protamine Kinase Protein Kinases Substrate Specificity Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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G D Amick, Z Damuni. 1992-03-16. Protamine kinase phosphorylates eukaryotic protein synthesis initiation factor 4E.. https://doi.org/10.1016/0006-291x(92)90499-b

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