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PubMed · 1374329

Simultaneous and iterative weighted regression analysis of toxicity tests using a microplate reader.

F Galgani·Y Cadiou·F Gilbert
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Abstract

A system is described for determination of LC50 or IC50 by an iterative process based on data obtained from a plate reader using a marine unicellular alga as a target species. The esterase activity of Tetraselmis suesica on fluorescein diacetate as a substrate was measured using a fluorescence titerplate. Simultaneous analysis of results was performed using an iterative process adopting the sigmoid function Y = y/1 (dose of toxicant/IC50)slope for dose-response relationships. IC50 (+/- SEM) was estimated (P less than 0.05). An application with phosalone as a toxicant is presented.

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Cholinesterase InhibitorsComputersEsterasesEukaryotaLethal Dose 50Organothiophosphorus CompoundsRegression AnalysisToxicologyWater Pollutants, ChemicalJournal Article

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BibTeXRIS

F Galgani, Y Cadiou, F Gilbert. 1992. Simultaneous and iterative weighted regression analysis of toxicity tests using a microplate reader.. https://doi.org/10.1016/0147-6513(92)90061-7

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