Identification of basic nuclear proteins by their boronate complex.

K Jobst; A Lakatos; A Horváth

doi:10.3109/10520299209110027

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Identification of basic nuclear proteins by their boronate complex.

K Jobst·A Lakatos·A Horváth

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Abstract

A new histochemical reaction for the identification of histone type basic proteins has been developed. Carbonyldiimidazol is used to activate the basic proteins of TCA-extracted nuclei, their m-aminophenylboronic acid complex is prepared, and the DNA-free, histone-containing nuclei are stained with toluidine blue at pH 5.5.

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Animals Boron Cell Nucleus Histocytochemistry Histones Imidazoles Mice Nuclear Proteins Paraffin Embedding Staining and Labeling Tolonium Chloride Journal Article Research Support, Non-U.S. Gov't

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K Jobst, A Lakatos, A Horváth. 1992. Identification of basic nuclear proteins by their boronate complex.. https://doi.org/10.3109/10520299209110027

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