A new epoxy embedment for electron microscopy.

J A FREEMAN; B O SPURLOCK

doi:10.1083/jcb.13.3.437

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PubMed · 13894888

A new epoxy embedment for electron microscopy.

J A FREEMAN·B O SPURLOCK

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Abstract

A new epoxy embedding mixture has been developed utilizing Maraglas 655 and Cardolite NC-513 with benzyldimethylamine (BDMA) as a curing agent. This epoxy mixture permits cellular preservation comparable to that obtained with Epon 812, ease of preparation of tissues, a wide range of miscibility, low viscosity, and, most important, ease of sectioning on a Porter-Blum microtome. In contrast to Epon-812-embedded tissues, Maraglas-Cardolite-embedded tissues can be sectioned in large dimensions with ease and consistent results without "chatter." No background granularity is detectable with high magnification study of Maraglas-Cardolite-embedded tissues. This epoxy is readily stained with lead hydroxide and is relatively stable in the electron beam.

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Electrons Epoxy Resins Microscopy Microscopy, Electron Microtomy Resins, Synthetic MICROSCOPY, ELECTRON MICROTOMY RESINS Journal Article

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J A FREEMAN, B O SPURLOCK. 1962. A new epoxy embedment for electron microscopy.. https://doi.org/10.1083/jcb.13.3.437

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