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Extracellular perfusion controller for electrophysiology.

J R Stimers
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Abstract

This paper describes the design and utility of a perfusion controller to facilitate the handling of multiple extracellular solutions. The controller consists of an interface box with both manual and computer controls and a set of solenoid valves. This provides remote handling of the switching of multiple solutions into an experimental chamber. It is described how this system can be used in whole-cell patch-clamp experiments to measure various ion channel currents as well as Na/K pump currents. Other possible uses are discussed along with suggestions for interfacing the perfusion controller with existing perfusion systems.

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AnimalsComputersElectrophysiologyHeartMyocardiumOuabainPerfusionRatsRats, Sprague-DawleySoftwareJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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J R Stimers. 1992. Extracellular perfusion controller for electrophysiology.. https://doi.org/10.1016/0165-0270(92)90023-7

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