PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 14066466

BACTERIAL NUCLEOTIDASES.

J KOHN·J L REIS
View original source ↗Export metadata ↓

Abstract

Kohn, J. (Queen Mary's Hospital, London, England) and J. L. Reis. Bacterial nucleotidases. J. Bacteriol. 86:713-716. 1963.-The 3- and 5- nucleotidase activity in various bacterial species was investigated. Both enzymes were found in bacterial extracts in varying proportions. The nucleotidases were found to be very active in Proteus vulgaris, in which organism they were studied in detail. The relative activities, the pH optima, and the effect of metal ions were investigated. It was concluded that bacterial 3- and 5-nucleotidases are distinct and separate enzymes.

Explore related subjects

Bacillus cereusClostridiumClostridium perfringensEscherichia coliHaemophilus influenzaeMagnesiumManganeseNickelNucleotidasesProteusPseudomonas aeruginosaResearchSalmonellaSalmonella typhimuriumSerratia marcescensShigella dysenteriaeStaphylococcusStreptococcus pneumoniaeZincBACILLUS CEREUSCLOSTRIDIUMCLOSTRIDIUM PERFRINGENSDIPLOCOCCUS PNEUMONIAEESCHERICHIA COLIEXPERIMENTAL LAB STUDYHAEMOPHILUS INFLUENZAEMAGNESIUMMANGANESENICKELNUCLEOTIDASESPROTEUSPSEUDOMONAS AERUGINOSASALMONELLASALMONELLA TYPHIMURIUMSERRATIA MARCESCENSSHIGELLA DYSENTERIAESTAPHYLOCOCCUSZINCJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

J KOHN, J L REIS. 1963. BACTERIAL NUCLEOTIDASES.. https://doi.org/10.1128/jb.86.4.713-716.1963

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2026

Assessing the threat of Bacillus cereus: From toxin characterization to modern detection strategies.

Bacillus cereus is a spore-forming pathogen responsible for both diarrheal and emetic foodborne illnesses worldwide. Its significance in food safety has received growing attention. Recent advances, including the discovery of novel virulence factors and the development of emerging detection technologies, have provided new insights into its pathogenic mechanisms and surveillance strategies. This review critically examines the global burden of B. cereus infections, and molecular mechanisms of its major virulence factors, and the performance characteristics of current detection knowledge gaps such as the viable-but-non-culturable state and regulatory blind spots for emetic toxins, and discuss unresolved challenges in clinical management. By integrating epidemiological, microbiological, and technological perspectives with critical lens, this review aims to provide a valuable reference for future research and food safety practices.

Bacillus cereus↗
PubMed2026

Development and application of a fast and efficient CRISPR/Cas12f -based genetic toolkit in Bacillus cereus GW-01.

Bacillus cereus GW-01, an efficient degrader of β-cypermethrin (β-CY), has a high safety profile and probiotic potential for regulating intestinal flora and fermented foods, which is difficult to genetically engineer for modification due to its restrictive modification system. This study successfully developed a CRISPR/enCas12f-based genome editing system, first selecting the plcR gene for proof-of-concept validation with 100% knockout efficiency. Subsequently, this system was utilized to delete the virulence gene nheABC in GW-01, yielding a safer probiotic strain. Compared with the wild-type strain GW-01, the probiotic-related indicators of the ΔnheABC mutant, including cell surface hydrophobicity, auto-aggregation ability and biofilm formation ability, were 80%, 90% and 2.9 (OD₅₉₅), respectively. There were no significant differences in these indicators between the mutant and the wild type. Meanwhile, the ΔnheABC mutant still maintained a high β-cypermethrin degradation efficiency of 80% at the concentration of 30 μg/mL. This work facilitates functional genomic research and genetic modification of Bacillus cereus GW-01. The established CRISPR/enCas12f system enables targeted gene deletion to explore gene functions and phenotypic mechanisms, and paves the way for its development into safe probiotics and excellent microbial chassis.

Bacillus cereus↗
PubMed2007

The use of flow cytometry to study the germination of Bacillus cereus endospores.

BACKGROUND: At present the study of endospore germination is conducted using microbiological methods which are slow and yield data based on the means of large heterogeneous populations. Flow cytometry (FCM) offers the potential to rapidly quantify and identify germination and outgrowth events for large numbers of individual endospores. METHODS: Standard methods were employed to arrest the germination of Bacillus cereus endospores at defined stages. Endospores were then stained with SYTO 9 alone or carboxyfluorescein diacetate (CFDA) together with Hoechst 33342 and analysed using FCM. Comparisons were made between FCM as a method to measure germination rate and standard microbiological techniques. RESULTS: Germinating endospores displayed increases in permeability to SYTO 9 and hydrolysis of CFDA compared with controls. Statistically significant correlations were found between the standard plate count method and both FCM methods for measuring the percentage of germinating and outgrowing endospores up to 75 min after addition of germinant. CONCLUSIONS: Using FCM, the percentage of germinating or outgrowing endospores at various time points during germination and/or outgrowth can be quantified. FCM with CFDA/Hoechst 33342 staining may be used to estimate overall germination rate, whereas FCM with SYTO 9 staining may be used to quantify ungerminated, germinating and outgrowing endospores.

Bacillus cereus↗