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PubMed · 14300936

COMPUTERS, PHYSICIANS, AND THE DIAGNOSTIC DECISION-MAKING PROCESS.

C A ROGERS·J L BALINTFY·A M GOLDMAN
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ComputersDecision MakingDiagnosisElectronic Data ProcessingHumansAUTOMATIC DATA PROCESSINGCOMPUTERSDIAGNOSISJournal Article

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C A ROGERS, J L BALINTFY, A M GOLDMAN. 1964. COMPUTERS, PHYSICIANS, AND THE DIAGNOSTIC DECISION-MAKING PROCESS.. https://doi.org/10.1177/001872086400600503

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Development of an integrated automation system with a magnetic bead-mediated nucleic acid purification device for genetic analysis and gene manipulation.

We have developed an integrated automation system for genetic analysis and gene manipulation. The system, SX-8G Plus, is equipped with an 8-nozzle dispensing unit, a thermal cycler, a cooled reagent reservoir, four tip storage racks, four microplate platforms, buffer reservoirs, an agarose gel electrophoresis unit, a power supply, a pump for exchanging electrophoresis buffer, and a CCD camera. Automation of nucleic acid extraction and purification, the most difficult step in automating genetic analysis and gene manipulation, was realized using magnetic beads with Magtration Technology, which we have previously developed for automating the handling of paramagnetic beads. Using this system, we could perform the automated separation and purification of DNA fragments by agarose gel electrophoresis starting from sample loading. The system would enable the automation of almost all procedures in genetic analysis and gene manipulation.

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