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Segment identification of a ligand binding with a protein receptor using multidimensional T1rho-, diffusion-filtered and diffusion-ordered NOESY experiments.

Hiroaki Utsumi·Hiroko Seki·Kentaro Yamaguchi·Mitsuru Tashiro
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Abstract

Multidimensional T1rho-, diffusion-filtered and diffusion-ordered NOESY techniques were applied to identify segments of a ligand binding with a protein receptor. These experiments can easily provide intermolecular NOEs of the complex, which are of great significance for characterizing the binding epitopes of a ligand. This information cannot be obtained by high-throughput 1D NMR experiments, used for determining the binding affinity, although multidimensional NMR experiments require more experiment time. The present results indicate that the current experiments using T1rho- and diffusion-edited techniques are very suitable for identifying segments of a ligand binding with a protein receptor in the drug discovery process.

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Binding SitesLigandsMagnetic Resonance SpectroscopyProtein BindingProteinsSalicylic AcidJournal Article

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Hiroaki Utsumi, Hiroko Seki, Kentaro Yamaguchi, Mitsuru Tashiro. 2003. Segment identification of a ligand binding with a protein receptor using multidimensional T1rho-, diffusion-filtered and diffusion-ordered NOESY experiments.. https://doi.org/10.2116/analsci.19.1441

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