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Visual cycle retinoid processing proteins are present in HEK293S cells.

Yumei Chen·Gennadiy Moiseyev·Bill X Wu·Jian-xing Ma·Rosalie K Crouch
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Abstract

In HEK293S cells expressing opsin, rhodopsin regenerates on addition of all-trans retinol. This study was to determine if key proteins in the retinal pigment epithelium (RPE) are present in these cells. Cellular retinoid binding protein, cellular retinoic-acid binding protein, RPE65, caveolin-1-alpha- and -beta-isoforms, interphotoreceptor retinoid binding protein, and 11-cis retinol dehydrogenase, but not lecithin:retinol acyltransferase (LRAT), were identified by Western blot analysis. LRAT transcripts were found by RT-PCR and Southern blot analysis. Small interference RNA specific to LRAT reduced ester formation, confirming that the enzyme is present. Therefore, HEK293S cells contain the functional components of the retinoid cycle found in the RPE.

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AcyltransferasesBlotting, SouthernBlotting, WesternCarrier ProteinsCaveolin 1CaveolinsCells, CulturedEye ProteinsHumansKidneyPigment Epithelium of EyeProtein Array AnalysisProteinsRNAReceptors, Retinoic AcidRetinoidsRetinol-Binding ProteinsReverse Transcriptase Polymerase Chain Reactioncis-trans-IsomerasesRetinol-Binding Proteins, InterstitialRetinoid IsomerohydrolaseJournal Article

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Yumei Chen, Gennadiy Moiseyev, Bill X Wu, Jian-xing Ma, Rosalie K Crouch. 2003. Visual cycle retinoid processing proteins are present in HEK293S cells.. https://doi.org/10.1016/j.visres.2003.08.001

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