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PubMed · 14616034

Method for patterning stretched DNA molecules on mica surfaces by soft lithography.

M Gad·Shigeru Sugiyama·Toshio Ohtani
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Abstract

Lambda DNA was stretched and patterned on mica surface using soft lithography. A highly diluted solution of amino propyl trimethoxy silane in hexane was deposited on a line patterned polydimethylsiloxane (PDMS) stamp. The functionalized stamp was then used to pick up DNA by molecular combing while the line patterns are parallel to the liquid surface. The stamp was then microcontact printed on freshly cleaved mica. We successfully obtained stretched DNA pattern on mica surface. DNA was found to be stretched in patterns perpendicular to those carved on the stamp. The stretched DNA population was large enough to be used for molecular biology mapping studies. Furthermore, the possibility of locating stretched DNA molecules in the desired position by stamping makes this method a good candidate for assembling non-semiconductor molecular devices.

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Aluminum SilicatesDNADimethylpolysiloxanesMicroscopy, Atomic ForceMicroscopy, FluorescenceNucleic Acid ConformationOligonucleotidesSiliconesJournal ArticleResearch Support, Non-U.S. Gov't

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M Gad, Shigeru Sugiyama, Toshio Ohtani. 2003. Method for patterning stretched DNA molecules on mica surfaces by soft lithography.. https://doi.org/10.1080/07391102.2003.10506934

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