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Laser-assisted microdissection applied to frozen surgical pathologic specimens - methodological aspects on RT-PCR.

Isabelle Saal·Anne Gustin·Katia Rombaut·Pilar Pelaez·Sandrine Rorive·Myriam Remmelink·Isabelle Salmon
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Abstract

Laser-assisted microdissection has become a unique technique for an accurate gene-expression profiling analysis in human tissues. The introduction of this approach requires the development of a reliable, efficient, and reproducible procedure for tissue processing. We report a systematic evaluation of the different relevant steps required to obtain sufficient quantity and good quality RNA for reverse transcriptase-polymerase chain reaction when using frozen surgical pathologic tissues as starting material. We propose an optimized and very efficient method with respect to time and effort that can easily be put into practice in research laboratory as well as in any pathology laboratory.

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ColonFrozen SectionsHumansLaser TherapyLungMicrodissectionNeoplasmsRNA, NeoplasmReverse Transcriptase Polymerase Chain ReactionThyroid GlandEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Isabelle Saal, Anne Gustin, Katia Rombaut, Pilar Pelaez, Sandrine Rorive, Myriam Remmelink, Isabelle Salmon. Laser-assisted microdissection applied to frozen surgical pathologic specimens - methodological aspects on RT-PCR.. https://doi.org/10.1111/j.1533-869x.2003.01106.x

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