Abscisic acid studied by fluorescent techniques.

B del Castillo; J Alvarez-Builla; C J Martinez-Honduvilla

PubMed · 147250

Abscisic acid studied by fluorescent techniques.

B del Castillo·J Alvarez-Builla·C J Martinez-Honduvilla

Abstract

Crude analytical abscisic acid (ABA) exhibited fluorescence (lambdaex 340, lambdaem 425 nm). The results demonstrated a relationship between fluorescence response and ABA concentration, in the range of 0.04-0.38 microgram/ml. Chromatographic purification increased the emission signal, and oxidation induced by adding a sulphuric acid: ethanol (1:1) mixture also increased and displaced it (lambdaex 450, lambdaem 495 nm). Using these properties, a fluorimetric method for identification and evaluation of ABA, had been developed, and used for inhibitor recognition in P. pinea seed coats.

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Abscisic Acid Biological Assay Chemical Phenomena Chemistry Cyclohexanecarboxylic Acids Seeds Spectrometry, Fluorescence Journal Article

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B del Castillo, J Alvarez-Builla, C J Martinez-Honduvilla. Abscisic acid studied by fluorescent techniques.. https://pubmed.ncbi.nlm.nih.gov/147250/

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